色噜噜精品一区二区三区,国产乱码在线精品可播放,麻豆AV无码精品一区二区,久久亚洲精精品中文字幕,久久亚洲精品国产精品,久久久久久精品免费免费999,9999国产精品欧美久久久久久,国产精品va在线播放我和闺蜜,精品久久久无码人妻中文字幕,国产亚洲美女精品久久久,99精品视频免费热播在线观看

The base medium for this cell line is MCDB-131 (Gibco cat # 10372-019). To make the complete medium add to 500 mL of the base medium:

• 56 mL Fetal Bovine Serum (FBS; ATCC 30-2020)

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with DPBS (ATCC catalog # 30-2200) to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
   Cultures can be established between 2.0 x 10⁴ and 4.0 x 10⁴ viable cells/cm².
6. Incubate cultures at 37°C.

Candal FJ, et al. BMEC-1: a human bone marrow microvascular endothelial cell line with primary cell characteristics. Microvasc Res 52(3):221-34, 1996. PubMed: 8954864