產(chǎn)品名稱 |
Enterovirus type 70 |
商品貨號 |
B162331 |
Classification |
Picornavirus, Human Enterovirus |
Agent |
Enterovirus type 70 |
Strain |
J670/71 |
Applications |
Enteric Research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen |
Storage Conditions |
-70°C or colder |
Comments |
Apathogenic for newborn mice. Some neurovirulence in cynomolgus monkeys that are inoculated intraspinally as well as intrathalamically. The virus is banded by gradient centrifugation in cesium chloride at a density of 1.34 g/mL. |
Effect on Host |
CPE, refractile rounding and sloughing |
Recommended Host |
WI-38 cells (ATCC® CCL-75™?)
|
Growth Conditions |
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 75% - 85% confluent (not 100% confluent).
Incubation: 1 to 5 days at 37°C, a 5% CO2 in air atmosphere is recommended |
Name of Depositor |
JL Melnick |
Source |
Conjunctival scrapings or swabs from patients in Japan with acute hemorrhagic conjunctivitis |
References |
Mirkovic RR, et al. Enterovirus type 70: the etiologic agent of pandemic acute haemorrhagic conjunctivitis. Bull. World Health Organ. 49: 341-346, 1973. PubMed: 4368683
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