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The cell-binding sequence and a potential N-linked glycosylation site are conserved in both human and rat.

Construct size (kb): 4.0

DNA: cDNA

Insert lengths(kb): 1.399999976158142

Tissue: osteosarcoma

Gene product: secreted phosphoprotein 1 (includes osteopontin)( 44kDa bone phosphoprotein, Secreted phosphoprotein-1 (osteopontin, bone sialoprotein), bone sialoprotein, osteopontin, early T lymphocyte activation, osteopontin) [OPN]

Alleles: A1, A1, A2, B1, A2, B1, B2, B2

Insert contains the following sites separated by (bp): (BamHI/BglII)- 272- AhaIII- 480- PvuII- 129- EcoRI- 209- XmnI- 116- HindIII- 61- SspI- 157- (BglII/BamHI). (BamHI and BglII sites destroyed in cloning.).

Restriction digests of the clone give the following sizes (kb): XbaI/PstI--2.75, 1.38; XbaI--4.0; PstI--4.0; EcoRI--3.5, 0.56; HindIII--3.6, 0.34.

The 5' EcoRI fragment of the clone detects genomic EcoRI fragments of 6.8 kb, 2.1 kb, and 0.9 kb in the human; 5.5 kb in the hamster; and 6.6 kb in the mouse. It also detects a human genomic BamHI fragment of 25 kb and KpnI fragment of 11.9 kb.

This probe is a full-length cDNA.

The human and rat polypeptide precursors are 65% identical. The cell-binding sequence and a potential N-linked glycosylation site are conserved in both human and rat.

Enzyme(s) not detecting polymorphism: EcoRI, HindIII, BamHI, XbaI, sacI, TaqI, PvuII, PstI, EcoRV, KpnI.