產(chǎn)品名稱 |
Mumps rubulavirus |
商品貨號(hào) |
B162814 |
Classification |
Paramyxoviridae, Rubulavirus |
Agent |
Mumps rubulavirus |
Strain |
MuV/Iowa.US/2006 (Genotype G) |
Common Name |
Mumps virus |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1.0 mL per vial |
Storage Conditions |
-70°C or colder |
Genome Sequenced Strain |
Mumps virus strain MuV-IA, complete genome
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Images |
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Effect on Host |
CPE, cell fusion and sloughing |
Recommended Host |
Vero E6 cells (ATCC® CRL-1586™) |
Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001-0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.
Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
Name of Depositor |
P Rota, CDC |
Source |
Isolated by oral swab from a human in Iowa, USA, 2006 |
References |
Boddicker JD, et al. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens. J Clin Microbiol. 45(9): 2902-2908, 2007. PubMed: 17652480
Xu P, et al. Rescue of wild-type mumps virus from a strain associated with recent outbreaks helps to define the role of the SH ORF in the pathogenesis of mumps virus. Virology 417(1): 126-136, 2011. PubMed: 21676427
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Cross References |
Nucleotide (GenBank) :
JN012242.1
Mumps virus strain MuV-IA, complete genome
Nucleotide (GenBank) :
DQ661745.1
Mumps virus strain MUM/Iowa.US/2006 small hydrophobic protein mRNA, complete cds
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