產(chǎn)品名稱 |
Influenza A virus (H3N2) |
商品貨號 |
B162985 |
Classification |
Orthomyxoviridae, Alphainfluenzavirus |
Agent |
Influenza A virus (H3N2) |
Strain |
A/Hong Kong/8/68 |
Applications |
Emerging infectious disease research Respiratory research Influenza Research |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Derived by adaptation from allantoic product ATCC® VR-544™ to MDCK cells (ATCC® CCL-34™). ATCC® VR-544™ and ATCC® VR-1679™ have not been compared for sequence or infectivity in chicken embryo culture and tissue culture. |
Effect on Host |
CPE, rounding and degeneration |
Recommended Host |
MDCK (ATCC® CCL-34) |
Growth Conditions |
Temperature: 35°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 35°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
Name of Depositor |
MT Coleman, Centers of Disease Control and Prevention; ATCC® |
Source |
Isolated from a patient in Hong Kong, 1968 |
References |
ASTM International Standard Test Method for Efficacy of Antimicrobial Agents Against Viruses in Suspension. West Conshohocken, PA (Reapproved 2002).
. . WHO Weekly Epid. Record 43: 401, 1968.
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