產(chǎn)品名稱 |
Felid herpesvirus 1 |
商品貨號(hào) |
B163249 |
Classification |
Herpesviridae, Varicellovirus |
Deposited As |
Feline rhinotracheitis virus |
Agent |
Felid herpesvirus 1 |
Strain |
FVRm (modified) |
Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Product Format |
frozen 1 mL per vial |
Storage Conditions |
-70°C or colder |
Images |
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Comments |
Derived by adaptation from primary cell culture product ATCC® VR-815™ to CRFK cells (ATCC® CCL-94™). ATCC® VR-815™ and ATCC® VR-1787™ have not been compared for sequence or infectivity in primary cell culture and tissue culture. Item is a vaccine strain. Antigenicity is specific for virus. |
Effect on Host |
CPE, cell rounding and sloughing |
Recommended Host |
CRFK (ATCC® CCL-94™) |
Growth Conditions |
Temperature: 31°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 31°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-5 days |
Name of Depositor |
EV Davis, Norden Laboratories, Inc. |
Source |
Throat swab from a 3 month old cat |
References |
Davis E. Feline viral rhinotracheitis vaccine and combination feline viral rhinotracheitis-calicivirus vaccine prepared therefrom. US Patent 4,031,204 dated Jun 21 1977
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