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Size (kb): 8.8999996185302730

Vector: REP3X (phagemid)

Promoters: Promoter for expression nmt1 (full strength)

Construction: pUC119

Marker(s):LEU2,ampR

Construct size (kb): 8.899999618530273

Features: marker(s): LEU2
marker(s): ampR
promoter for expression: nmt1 (full strength)
replicon: ars1
replicon: f1
replicon: pMB1
MCS: Xho...SmaI

expression vector

Restriction digests of the clone give the following sizes (kb): HindIII--5.6, 2.3, 1.1; EcoRI--4.3, 3.5, 1.2; XhoI--9.0.

REP3X (ATCC 87603) and REP4X (ATCC 87604) contain full strength nmt1 promoter whereas REP41X (ATCC 87605) and REP42X (ATCC 87607) with medium strength (nmt1*) and REP81X (ATCC 87606) and REP82X (ATCC 87608) with low strength (nmt1**)

due to mutation in the TATA box by Basi et al. (Gene 123:131-136, 1993).

REP3X (ATCC 87603) and REP4X (ATCC 87604) differ in that REP3X contains LEU2 (2.2 kb) and REP4X contains ura4+ (1.8 kb).

REP3X was constructed by removing an ATG within the polylinker of an expression vector REP3, which contains the thiamine-inducible nmt1 promoter (J. Biol. Chem. 265:10857-10862, 1993), by insertion of a XhoI linker into Ball and SalI sites.

The SalI site is preserved.

Temperature: 37.0°C

component of:ATCC 87685