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pTHH37 [RW-23]
pTHH37 [RW-23]
規(guī)格:
貨期:
編號(hào):B239590
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTHH37 [RW-23]
商品貨號(hào) B239590
Designations pTHH37 [RW-23]
Species Homo sapiens, human
Depositors RL White, Y Nakamura
Applications
RFLPs observed under normal hybridization stringency; washes 0.1X SSC, 0.1% SDS, 65 C.
Vector
Construct size (kb): 5.099999904632568
Insert
DNA: genomic
Insert lengths(kb): 2.200000047683716
Gene product: DNA Segment, single copy [D14S16]
Alleles: A1, A2, A3
Insert Size (kb) 2.200
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1; HindIII--4.2, 0.98; BamHI--5.0; AvaI--3.1, 2.0.
PstI detects a 3 allele polymorphism with bands between 2-3 kb. MspI and TaqI detect the same polymorphism. Codominant segregation has been shown in 60 families.
RFLPs observed under normal hybridization stringency; washes 0.1X SSC, 0.1% SDS, 65 C.
Enzyme(s) not detecting polymorphism: RsaI.
References

Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872

Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (pTHH37) on chromosome 14 [D14S16]. Nucleic Acids Res. 16: 3593, 1988. PubMed: 2897662

Peter J O'Connell, personal communication

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