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Construct size (kb): 8.600000381469727

DNA: cDNA

Insert lengths(kb): 4.400000095367432

Gene product: leukotoxin [lktA]

The insert includes the following sites, numbered beginning at -469 for the EcoRV site: PvuI--158, HincII--227, BglII--346, PvuII--1252, PstI--1650, EcoRV--2683, XbaI--3920.

Restriction digests of the clone give the following sizes (kb): SalI--9.4; EcoRV/SalI--4.4, 3.4, 1.3; EcoRV--5.8, 3.2.

The insert includes 2 open reading frames; one for lktC encoding a protein of 19.8 kDa and one for lktA encoding a protein of 101.9 kDa. HB101 transformed with the plasmid shows the 100 kDa protein on western blots, but not the 20 kDa protein.