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YCF3
YCF3
規(guī)格:
貨期:
編號:B241024
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 YCF3
商品貨號 B241024
Designations YCF3
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 7.0999999046325680
Vector: YCF3 (plasmid)
Construction: YRp14
Marker(s):URA3,ampR,SUP11
Construct size (kb): 7.099999904632568
Features: marker(s): ampR, URA3, SUP11
replicon: pMB1
Applications
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.1; HindIII--7.1; BamHI--7.1; HindIII/BglII--7.1.
The sequence to be mapped is cloned into the MCS of both YCF3 (ATCC 77155) and YCF4 (ATCC 77156) (both orientations being produced). NotI is used to linearize the constructs, which are transformed independently into YPH49.
YCF3 is used to recover sequences proximal to the mapping sequence.
Shuttle chromosome fragmentation vector.
Constructed by replacing the 346 bp HindIII/BamHI fragment from YRp14 with a 1.5 kb HindIII/BglII fragment containing the telomere-adjacent sequence (Y') and a polylinker. EcoRI and HindIII sites were removed.
The order of the major features in this plasmid is: Y' - MCS - SalI - URA3 - SUP11 - rep - pMB1 ori - bla.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Vollrath D, et al. Physical mapping of large DNA by chromosome fragmentation. Proc. Natl. Acad. Sci. USA 85: 6027-6031, 1988. PubMed: 3045811

Gerring SL, et al. Positional mapping of genes by chromosome blotting and chromosome fragmentation. Methods Enzymol. 194: 57-77, 1991. PubMed: 2005810

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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