Restriction digests of the clone give the following sizes (kb): EcoRI--12.0; HindIII--8.0, 2.9; SalI--12.0; BamHI--8.5, 3.1; PstI--12.0. Light emission can be detected after promoter insertion by adding dodecyl aldehyde. A promoter cloning, Escherichia coli/Bacillus subtilis shuttle vector. |