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Distribution host: Escherichia coli DH5alpha

Size (kb): 4.6999998092651370

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: SmaI closer to BamHI site
Vector end: SmaI
Cloning sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Polylinker sites: KpnI Eco0109I ApaI DraII XhoI SalI HincII AccI ClaI Bsp106I
HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI
SacII BstXI SacI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references:

Vector: pBluc* (phagemid)

Promoters: Promoter for in vitro transcription T7 (phi10)

Construction: pBluescript SK+, pUHC131-1

Marker(s):ampR

Construct size (kb): 4.699999809265137

Features: insert detection: lacZ'
marker(s): ampR
promoter for in vitro transcription: T3
promoter for in vitro transcription: T7 (phi10)
replicon: f1
replicon: pMB1
MCS: Kpn...SacI
coding sequence: luc*

contains easily purifiable cassette(s) for construction luciferase, modified

Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.0, 1.7; BamHI/BstEII--4.0, 0.7; NcoI--4.7.

One of two vectors (ATCC 87108 and 87109) containing a luciferase gene altered to conserve amino acid sequence and function but to remove internal restriction sites to facilitate excision.

These constructs differ in their orientation. The sites listed in the polylinker (MCS) can be used to excise the gene for insertion into another plasmid.

Temperature: 37.0°C